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brd4 expression (Genecopoeia)

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Genecopoeia brd4 expression

FIGURE 1 MiR-152-5p was downregulated and <t>bromodomain</t> <t>containing</t> <t>4</t> <t>(BRD4)</t> was upregulated in cervical carcinoma tissue and adjacent normal tissues. (A) The expression of miR-152-5p was detected by quantitative real-time PCR (qRT-PCR) in cervical carcinoma tissue and adjacent normal tissues. (B) The expression of <t>BRD4</t> was detected by qRT-PCR. (C) The correlation was analyzed by Pearson's analysis. ***p < 0.001

Brd4 Expression, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brd4 expression - by Bioz Stars, 2026-10

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1) Product Images from "Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis."

Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.

Journal: The Kaohsiung journal of medical sciences

doi: 10.1002/kjm2.12370

FIGURE 1 MiR-152-5p was downregulated and bromodomain containing 4 (BRD4) was upregulated in cervical carcinoma tissue and adjacent normal tissues. (A) The expression of miR-152-5p was detected by quantitative real-time PCR (qRT-PCR) in cervical carcinoma tissue and adjacent normal tissues. (B) The expression of BRD4 was detected by qRT-PCR. (C) The correlation was analyzed by Pearson's analysis. ***p < 0.001

Figure Legend Snippet: FIGURE 1 MiR-152-5p was downregulated and bromodomain containing 4 (BRD4) was upregulated in cervical carcinoma tissue and adjacent normal tissues. (A) The expression of miR-152-5p was detected by quantitative real-time PCR (qRT-PCR) in cervical carcinoma tissue and adjacent normal tissues. (B) The expression of BRD4 was detected by qRT-PCR. (C) The correlation was analyzed by Pearson's analysis. ***p < 0.001

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

FIGURE 2 Apigenin inhibited the proliferation and invasion of cervical carcinoma cells by regulating miR-152-5p expression. (A) Quantitative real-time PCR (qRT-PCR) was conducted to determine the expression of miR-152-5p and bromodomain containing 4 (BRD4) in cell lines of HOSEpiC, C33A, Hela, SiHa, and CaSki. (B) qRT-PCR was used to detect the transfection efficiency of miR-152-5p in Hela and CaSki cells transfected with miR- 152-5p inhibitor and the NC inhibitor. (C) MTT was conducted to evaluate the proliferation in Hela and CaSki cells treated with apigenin, cells treated with apigenin and transfected with miR-152-5p inhibitor, cells treated with apigenin and transfected with NC, and negative control, respectively. (D) Transwell invasion assay was applied to determine the cell invasion. ***p < 0.001

Figure Legend Snippet: FIGURE 2 Apigenin inhibited the proliferation and invasion of cervical carcinoma cells by regulating miR-152-5p expression. (A) Quantitative real-time PCR (qRT-PCR) was conducted to determine the expression of miR-152-5p and bromodomain containing 4 (BRD4) in cell lines of HOSEpiC, C33A, Hela, SiHa, and CaSki. (B) qRT-PCR was used to detect the transfection efficiency of miR-152-5p in Hela and CaSki cells transfected with miR- 152-5p inhibitor and the NC inhibitor. (C) MTT was conducted to evaluate the proliferation in Hela and CaSki cells treated with apigenin, cells treated with apigenin and transfected with miR-152-5p inhibitor, cells treated with apigenin and transfected with NC, and negative control, respectively. (D) Transwell invasion assay was applied to determine the cell invasion. ***p < 0.001

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Transwell Invasion Assay

FIGURE 3 Apigenin inhibited epithelial–mesenchymal transition (EMT) of cervical carcinoma cells by regulating miR- 152-5p expression. (A) Quantitative real- time PCR (qRT-PCR) was used to calculate the expression of miR-152-5p and bromodomain containing 4 (BRD4). (B) Western blotting was conducted to detect the protein level of BRD4, E- cadherin, N-cadherin, and MMP9. ***p < 0.001

Figure Legend Snippet: FIGURE 3 Apigenin inhibited epithelial–mesenchymal transition (EMT) of cervical carcinoma cells by regulating miR- 152-5p expression. (A) Quantitative real- time PCR (qRT-PCR) was used to calculate the expression of miR-152-5p and bromodomain containing 4 (BRD4). (B) Western blotting was conducted to detect the protein level of BRD4, E- cadherin, N-cadherin, and MMP9. ***p < 0.001

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

FIGURE 4 Apigenin suppressed the proliferation and invasion of Hela and CaSki cells through regulating bromodomain containing 4 (BRD4) expression. (A) Quantitative real-time PCR (qRT-PCR) was used to detect the transfection efficiency of BRD4 in Hela and CaSki cells transfected with pcDNA3.1-BRD4 and the negative control (NC) control. (B) MTT was conducted to evaluate the proliferation of Hela and CaSki cells treated with apigenin, cells treated with apigenin and transfected with pcDNA3.1-BRD4, cells treated with apigenin and transfected with NC, and negative control, respectively. (C) Transwell was applied to determine the cell invasion. ***p < 0.001

Figure Legend Snippet: FIGURE 4 Apigenin suppressed the proliferation and invasion of Hela and CaSki cells through regulating bromodomain containing 4 (BRD4) expression. (A) Quantitative real-time PCR (qRT-PCR) was used to detect the transfection efficiency of BRD4 in Hela and CaSki cells transfected with pcDNA3.1-BRD4 and the negative control (NC) control. (B) MTT was conducted to evaluate the proliferation of Hela and CaSki cells treated with apigenin, cells treated with apigenin and transfected with pcDNA3.1-BRD4, cells treated with apigenin and transfected with NC, and negative control, respectively. (C) Transwell was applied to determine the cell invasion. ***p < 0.001

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Control

FIGURE 5 Apigenin inhibited epithelial–mesenchymal transition (EMT) of Hela and CaSki cells through regulating bromodomain containing 4 (BRD4) expression. (A) Quantitative real-time PCR (qRT-PCR) was used to calculate the expression of BRD4. (B) Western blotting was conducted to detect the protein level of BRD4, E-cadherin, N- cadherin, and MMP9. ***p < 0.001

Figure Legend Snippet: FIGURE 5 Apigenin inhibited epithelial–mesenchymal transition (EMT) of Hela and CaSki cells through regulating bromodomain containing 4 (BRD4) expression. (A) Quantitative real-time PCR (qRT-PCR) was used to calculate the expression of BRD4. (B) Western blotting was conducted to detect the protein level of BRD4, E-cadherin, N- cadherin, and MMP9. ***p < 0.001

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

FIGURE 6 miR-152-5p could directly bind to bromodomain containing 4 (BRD4) in Hela and CaSki cells. (a) The binding site of miR-152-5p with BRD4 was predicted according to TargetScan (http://www. targetscan.org/).(B) Quantitative real-time PCR (qRT-PCR) was used to detect the transfection efficiency of miR-152-5p in Hela and CaSki cells transfected with miR-152-5p mimics and the negative control (NC) mimics. (C) Targeting relationship between miR-152-5p and BRD4 was identified by luciferase reporter assay. ***p < 0.001

Figure Legend Snippet: FIGURE 6 miR-152-5p could directly bind to bromodomain containing 4 (BRD4) in Hela and CaSki cells. (a) The binding site of miR-152-5p with BRD4 was predicted according to TargetScan (http://www. targetscan.org/).(B) Quantitative real-time PCR (qRT-PCR) was used to detect the transfection efficiency of miR-152-5p in Hela and CaSki cells transfected with miR-152-5p mimics and the negative control (NC) mimics. (C) Targeting relationship between miR-152-5p and BRD4 was identified by luciferase reporter assay. ***p < 0.001

Techniques Used: Binding Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Luciferase, Reporter Assay

FIGURE 7 Apigenin suppressed proliferation and invasion by regulating miR-152-5p/bromodomain containing 4 (BRD4) axis in Hela and CaSki cells. (A) Quantitative real-time PCR (qRT-PCR) was used to detect the transfection efficiency of BRD4 in Hela and CaSki cells transfected with si-BRD4 and the negative control (NC) control. (B) MTT was conducted to determine the proliferation of cells treated with apigenin and transfected with miR-152-5p inhibitor, cells treated with apigenin and co-transfected with miR-152-5p inhibitor and si-NC, cells treated with apigenin and co-transfected with miR-152-5p inhibitor and si-BRD4, and the control. (C) Transwell invasion assay was performed to evaluate the cell invasion. ***p < 0.001

Figure Legend Snippet: FIGURE 7 Apigenin suppressed proliferation and invasion by regulating miR-152-5p/bromodomain containing 4 (BRD4) axis in Hela and CaSki cells. (A) Quantitative real-time PCR (qRT-PCR) was used to detect the transfection efficiency of BRD4 in Hela and CaSki cells transfected with si-BRD4 and the negative control (NC) control. (B) MTT was conducted to determine the proliferation of cells treated with apigenin and transfected with miR-152-5p inhibitor, cells treated with apigenin and co-transfected with miR-152-5p inhibitor and si-NC, cells treated with apigenin and co-transfected with miR-152-5p inhibitor and si-BRD4, and the control. (C) Transwell invasion assay was performed to evaluate the cell invasion. ***p < 0.001

Techniques Used: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Control, Transwell Invasion Assay

FIGURE 8 Apigenin suppressed epithelial–mesenchymal transition (EMT) by regulating miR-152-5p/bromodomain containing 4 (BRD4) axis in Hela and CaSki cells. (A) Quantitative real-time PCR (qRT-PCR) was used to calculate the expression of BRD4. (B) Western blotting was conducted to detect the protein level of BRD4, E-cadherin, N-cadherin, and MMP9. ***p < 0.001

Figure Legend Snippet: FIGURE 8 Apigenin suppressed epithelial–mesenchymal transition (EMT) by regulating miR-152-5p/bromodomain containing 4 (BRD4) axis in Hela and CaSki cells. (A) Quantitative real-time PCR (qRT-PCR) was used to calculate the expression of BRD4. (B) Western blotting was conducted to detect the protein level of BRD4, E-cadherin, N-cadherin, and MMP9. ***p < 0.001

Techniques Used: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Western Blot

Control:

Article Title: BRD4 regulates key transcription factors that drive epithelial-mesenchymal transition in castration-resistant prostate cancer
Article Snippet: pho-Smad3 (Abcam). Additional information is available in a Supplementary File . Lentivirus-mediated eGFP-BRD4 expressing plasmid (EX-E0102-Lv122) and control vector (EX-NEG-Lv242) were purchased from GeneCopoeia. ON-Targetplus Human BET and Non-Targeting (scramble) SMARTpool siRNAs were purchased from Dharmacon. Additional information is available in a Supplementary File . Cell pellets were lysed

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Journal: The Kaohsiung journal of medical sciences

Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.

doi: 10.1002/kjm2.12370

Figure Lengend Snippet: FIGURE 1 MiR-152-5p was downregulated and bromodomain containing 4 (BRD4) was upregulated in cervical carcinoma tissue and adjacent normal tissues. (A) The expression of miR-152-5p was detected by quantitative real-time PCR (qRT-PCR) in cervical carcinoma tissue and adjacent normal tissues. (B) The expression of BRD4 was detected by qRT-PCR. (C) The correlation was analyzed by Pearson's analysis. ***p < 0.001

Article Snippet: For the detection of BRD4 expression, PrimeScript RT reagent Kit (QP015, GeneCopoeia, USA) was used for reverse transcription, and SYBR Premix Ex Taq (TaKaRa Biotechnology, Dalian, China) on SYBRTM Green PCR Master Mix (Applied Biosystems, Foster City, CA) were applied for the evaluation.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

Journal: The Kaohsiung journal of medical sciences

Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.

doi: 10.1002/kjm2.12370

Figure Lengend Snippet: FIGURE 2 Apigenin inhibited the proliferation and invasion of cervical carcinoma cells by regulating miR-152-5p expression. (A) Quantitative real-time PCR (qRT-PCR) was conducted to determine the expression of miR-152-5p and bromodomain containing 4 (BRD4) in cell lines of HOSEpiC, C33A, Hela, SiHa, and CaSki. (B) qRT-PCR was used to detect the transfection efficiency of miR-152-5p in Hela and CaSki cells transfected with miR- 152-5p inhibitor and the NC inhibitor. (C) MTT was conducted to evaluate the proliferation in Hela and CaSki cells treated with apigenin, cells treated with apigenin and transfected with miR-152-5p inhibitor, cells treated with apigenin and transfected with NC, and negative control, respectively. (D) Transwell invasion assay was applied to determine the cell invasion. ***p < 0.001

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Transwell Invasion Assay

Journal: The Kaohsiung journal of medical sciences

Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.

doi: 10.1002/kjm2.12370

Figure Lengend Snippet: FIGURE 3 Apigenin inhibited epithelial–mesenchymal transition (EMT) of cervical carcinoma cells by regulating miR- 152-5p expression. (A) Quantitative real- time PCR (qRT-PCR) was used to calculate the expression of miR-152-5p and bromodomain containing 4 (BRD4). (B) Western blotting was conducted to detect the protein level of BRD4, E- cadherin, N-cadherin, and MMP9. ***p < 0.001

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

Journal: The Kaohsiung journal of medical sciences

Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.

doi: 10.1002/kjm2.12370

Figure Lengend Snippet: FIGURE 4 Apigenin suppressed the proliferation and invasion of Hela and CaSki cells through regulating bromodomain containing 4 (BRD4) expression. (A) Quantitative real-time PCR (qRT-PCR) was used to detect the transfection efficiency of BRD4 in Hela and CaSki cells transfected with pcDNA3.1-BRD4 and the negative control (NC) control. (B) MTT was conducted to evaluate the proliferation of Hela and CaSki cells treated with apigenin, cells treated with apigenin and transfected with pcDNA3.1-BRD4, cells treated with apigenin and transfected with NC, and negative control, respectively. (C) Transwell was applied to determine the cell invasion. ***p < 0.001

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Control

Journal: The Kaohsiung journal of medical sciences

Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.

doi: 10.1002/kjm2.12370

Figure Lengend Snippet: FIGURE 5 Apigenin inhibited epithelial–mesenchymal transition (EMT) of Hela and CaSki cells through regulating bromodomain containing 4 (BRD4) expression. (A) Quantitative real-time PCR (qRT-PCR) was used to calculate the expression of BRD4. (B) Western blotting was conducted to detect the protein level of BRD4, E-cadherin, N- cadherin, and MMP9. ***p < 0.001

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

Journal: The Kaohsiung journal of medical sciences

Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.

doi: 10.1002/kjm2.12370

Figure Lengend Snippet: FIGURE 6 miR-152-5p could directly bind to bromodomain containing 4 (BRD4) in Hela and CaSki cells. (a) The binding site of miR-152-5p with BRD4 was predicted according to TargetScan (http://www. targetscan.org/).(B) Quantitative real-time PCR (qRT-PCR) was used to detect the transfection efficiency of miR-152-5p in Hela and CaSki cells transfected with miR-152-5p mimics and the negative control (NC) mimics. (C) Targeting relationship between miR-152-5p and BRD4 was identified by luciferase reporter assay. ***p < 0.001

Techniques: Binding Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Luciferase, Reporter Assay

Journal: The Kaohsiung journal of medical sciences

Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.

doi: 10.1002/kjm2.12370

Figure Lengend Snippet: FIGURE 7 Apigenin suppressed proliferation and invasion by regulating miR-152-5p/bromodomain containing 4 (BRD4) axis in Hela and CaSki cells. (A) Quantitative real-time PCR (qRT-PCR) was used to detect the transfection efficiency of BRD4 in Hela and CaSki cells transfected with si-BRD4 and the negative control (NC) control. (B) MTT was conducted to determine the proliferation of cells treated with apigenin and transfected with miR-152-5p inhibitor, cells treated with apigenin and co-transfected with miR-152-5p inhibitor and si-NC, cells treated with apigenin and co-transfected with miR-152-5p inhibitor and si-BRD4, and the control. (C) Transwell invasion assay was performed to evaluate the cell invasion. ***p < 0.001

Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Control, Transwell Invasion Assay

Journal: The Kaohsiung journal of medical sciences

Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.

doi: 10.1002/kjm2.12370

Figure Lengend Snippet: FIGURE 8 Apigenin suppressed epithelial–mesenchymal transition (EMT) by regulating miR-152-5p/bromodomain containing 4 (BRD4) axis in Hela and CaSki cells. (A) Quantitative real-time PCR (qRT-PCR) was used to calculate the expression of BRD4. (B) Western blotting was conducted to detect the protein level of BRD4, E-cadherin, N-cadherin, and MMP9. ***p < 0.001

Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Western Blot

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brd4 expression (Genecopoeia)

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1) Product Images from "Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis."

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